| |
| Names | |
|---|---|
| Other names
6-FAM
| |
| Identifiers | |
3D model (
JSmol)
|
|
| ChEBI | |
| ChEMBL | |
| ChemSpider | |
| ECHA InfoCard | 100.164.295 |
PubChem
CID
|
|
| UNII | |
CompTox Dashboard (
EPA)
|
|
| |
| |
| Properties | |
| C21H12O7 | |
| Molar mass | 376.320 g·mol−1 |
| Hazards | |
| GHS labelling: | |
| |
| Warning | |
| H315, H319, H335 | |
| P261, P305+P351+P338 | |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
| |
6-Carboxyfluorescein (6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm. A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents. It is used in the sequencing of nucleic acids and in the labeling of nucleotides.
Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5(6)-carboxyfluorescein.
The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading. [1] It can be incorporated into liposomes, and allows for the tracking of liposomes as they pass through the body. In addition, carboxyfluorescein has been used to track division of cells. [2] In vascular plants, 5(6)-carboxyfluorescein can be used as a symplastic tracer. It is able to move through the phloem due to its structural similarity to sucrose. [3] It is typically loaded into the leaves in order to gain access to the phloem. [4] [5] This can be done by scraping, cutting, or weakening the leaf’s cuticle with an herbicide.
Popular derivatives for cell tracing purposes are carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and carboxyfluorescein succinimidyl ester (CFSE).
- ^ Molecular Imaging Products Company (2005-08-26). "5-(and-6)-Carboxyfluorescein (5-(and-6)- FAM,mixed isomer) 100mg". Retrieved 2006-08-26.
- ^ Parish, Christopher (December 1999). "Fluorescent dyes for lymphocyte migration and proliferation studies". Immunology and Cell Biology. 77 (6). Blackwell Synergy: 499–508. doi: 10.1046/j.1440-1711.1999.00877.x. PMID 10571670. S2CID 2194612. Retrieved 2006-08-26.
- ^ Schulz, Alexander; Liesche, Johannes (2013). "Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders". Frontiers in Plant Science. 4: 207. doi: 10.3389/fpls.2013.00207. ISSN 1664-462X. PMC 3685819. PMID 23802006.
- ^ Martens, Helle Juel; Schulz, Alexander; Rademaker, Hanna; Andersen, Signe R.; Binczycki, Piotr; Gao, Chen; Liesche, Johannes (2019-04-01). "Direct Comparison of Leaf Plasmodesma Structure and Function in Relation to Phloem-Loading Type". Plant Physiology. 179 (4): 1768–1778. doi: 10.1104/pp.18.01353. ISSN 0032-0889. PMC 6446768. PMID 30723179.
- ^ Zambryski, P. C.; Hempel, F. D.; Barella, S.; Gisel, A. (1999-05-01). "Temporal and spatial regulation of symplastic trafficking during development in Arabidopsis thaliana apices". Development. 126 (9): 1879–1889. doi: 10.1242/dev.126.9.1879. ISSN 0950-1991. PMID 10101122.
|
| |
| Names | |
|---|---|
| Other names
6-FAM
| |
| Identifiers | |
3D model (
JSmol)
|
|
| ChEBI | |
| ChEMBL | |
| ChemSpider | |
| ECHA InfoCard | 100.164.295 |
PubChem
CID
|
|
| UNII | |
CompTox Dashboard (
EPA)
|
|
| |
| |
| Properties | |
| C21H12O7 | |
| Molar mass | 376.320 g·mol−1 |
| Hazards | |
| GHS labelling: | |
|
| |
| Warning | |
| H315, H319, H335 | |
| P261, P305+P351+P338 | |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
| |
6-Carboxyfluorescein (6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm. A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents. It is used in the sequencing of nucleic acids and in the labeling of nucleotides.
Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5(6)-carboxyfluorescein.
The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading. [1] It can be incorporated into liposomes, and allows for the tracking of liposomes as they pass through the body. In addition, carboxyfluorescein has been used to track division of cells. [2] In vascular plants, 5(6)-carboxyfluorescein can be used as a symplastic tracer. It is able to move through the phloem due to its structural similarity to sucrose. [3] It is typically loaded into the leaves in order to gain access to the phloem. [4] [5] This can be done by scraping, cutting, or weakening the leaf’s cuticle with an herbicide.
Popular derivatives for cell tracing purposes are carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and carboxyfluorescein succinimidyl ester (CFSE).
- ^ Molecular Imaging Products Company (2005-08-26). "5-(and-6)-Carboxyfluorescein (5-(and-6)- FAM,mixed isomer) 100mg". Retrieved 2006-08-26.
- ^ Parish, Christopher (December 1999). "Fluorescent dyes for lymphocyte migration and proliferation studies". Immunology and Cell Biology. 77 (6). Blackwell Synergy: 499–508. doi: 10.1046/j.1440-1711.1999.00877.x. PMID 10571670. S2CID 2194612. Retrieved 2006-08-26.
- ^ Schulz, Alexander; Liesche, Johannes (2013). "Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders". Frontiers in Plant Science. 4: 207. doi: 10.3389/fpls.2013.00207. ISSN 1664-462X. PMC 3685819. PMID 23802006.
- ^ Martens, Helle Juel; Schulz, Alexander; Rademaker, Hanna; Andersen, Signe R.; Binczycki, Piotr; Gao, Chen; Liesche, Johannes (2019-04-01). "Direct Comparison of Leaf Plasmodesma Structure and Function in Relation to Phloem-Loading Type". Plant Physiology. 179 (4): 1768–1778. doi: 10.1104/pp.18.01353. ISSN 0032-0889. PMC 6446768. PMID 30723179.
- ^ Zambryski, P. C.; Hempel, F. D.; Barella, S.; Gisel, A. (1999-05-01). "Temporal and spatial regulation of symplastic trafficking during development in Arabidopsis thaliana apices". Development. 126 (9): 1879–1889. doi: 10.1242/dev.126.9.1879. ISSN 0950-1991. PMID 10101122.