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Names | |
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IUPAC name
4-(2-Formylquinoline-3-carbonyl)benzoic acid
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Other names
CBQCA, ATTO-TAG CBQCA
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Identifiers | |
3D model (
JSmol)
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PubChem
CID
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CompTox Dashboard (
EPA)
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Properties | |
C18H11NO4 | |
Molar mass | 305.289 g·mol−1 |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
|
3-(4-Carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) is a fluorogenic amine labeling dye that is not fluorescent itself, but covalently reacts with primary amines to form fluorescent products. [1] It was first reported in 1991. [2]
CBQCA is largely used in the context of quantifying peptides or proteins. Either cyanide or thiols are required as a co-substrate in the fluorogenic reaction, although thiols also react with and mask the CBQCA aldehyde thereby preventing the fluorogenic reaction against the targeted primary amines. [1] Once bound to protein the excitation wavelength is 465 nm (blue) and the emission wavelength is ~550 nm (green). [3]
CBQCA, which is non-fluorescent, reacts with primary amines in the presence of cyanide to form fluorescent products:
![]() | |
Names | |
---|---|
IUPAC name
4-(2-Formylquinoline-3-carbonyl)benzoic acid
| |
Other names
CBQCA, ATTO-TAG CBQCA
| |
Identifiers | |
3D model (
JSmol)
|
|
PubChem
CID
|
|
CompTox Dashboard (
EPA)
|
|
| |
| |
Properties | |
C18H11NO4 | |
Molar mass | 305.289 g·mol−1 |
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
|
3-(4-Carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) is a fluorogenic amine labeling dye that is not fluorescent itself, but covalently reacts with primary amines to form fluorescent products. [1] It was first reported in 1991. [2]
CBQCA is largely used in the context of quantifying peptides or proteins. Either cyanide or thiols are required as a co-substrate in the fluorogenic reaction, although thiols also react with and mask the CBQCA aldehyde thereby preventing the fluorogenic reaction against the targeted primary amines. [1] Once bound to protein the excitation wavelength is 465 nm (blue) and the emission wavelength is ~550 nm (green). [3]
CBQCA, which is non-fluorescent, reacts with primary amines in the presence of cyanide to form fluorescent products: